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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two extra splice variants were being documented but in depth nucleotide sequence information is not nevertheless accessible.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally contains the epitope acknowledged with the antibody.
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This manual outlines additional probable optimization of mobile and immunoassay parameters to make sure the very best effects are...
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This guidebook outlines more attainable optimization of mobile and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin loved ones, whose associates are characterized by a dramatic periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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